The device for the treadmill test consists mainly of a rolling conveyor belt. The rear wall of the conveyor belt is equipped with stimulation electrodes and sound generating devices, and the stimulation devices of each channel are independent of each other. During the test, the rodents did running exercise training on a running platform with a set speed. If the animal refuses to move or moves at a speed lower than the experimental speed it will backtrack on the track and touch the stimulation device. Stronger electrical or acoustic stimuli will force the experimental animal to move at the speed of the running platform.

Fig. 1 Treadmill test in mice.

Experimental Flow

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• Pre-lab preparation and animal grouping.
• Before formal training, the animals are released to the track for a period of acclimatization.
• Place the experimental animals in the corresponding runway according to the number, and set the parameters such as the time, speed, and shock intensity of the treadmill.
• Record the corresponding parameter indicators during the training of experimental animals.