cDNA Synthesis
cDNA synthesis (complementary DNA synthesis) is the process of transforming RNA molecules existing in samples into DNA in complementary sequences. cDNA synthesis enables researchers to convert RNA into stable cDNA, which can be used for downstream applications such as PCR, microarray, and next-generation sequencing. By studying the amount of cDNA produced by genes, researchers can determine how much mRNA is present in the original sample and determine the expression level of specific genes.
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