Introduction to Synthetic DNA Library Construction

Synthetic DNA library refers to a collection of DNA fragments stored in host microorganisms by molecular cloning. Each fragment can be stored in a host organism separately. By rapidly and accurately replicating DNA in host organisms and producing detectable quantities, we can have quick access to many different sequences of interest with ease and efficiency. Synthetic DNA libraries enable us to achieve gene synthesis and cloning faster, more intelligent, and more reliable.

Synthetic DNA library provides a more accurate and effective approach for understanding and studying genetics. The design and construction of DNA libraries play an important role in identifying novel gene regulatory sequences or the minimal domains that are critical to protein structure and functions. Synthetic DNA libraries can also be used to construct genetic circuits of biosynthesis pathways for metabolic engineering.